Click image to view full size image
This kit utilizes isothermal nucleic acid amplification technology that operates at room temperature and a constant temperature range (typically 39℃–42℃). The process begins with reverse transcriptase synthesizing complementary DNA (cDNA) from RNA templates using specific primers. The complex formed by recombinase and primers, aided by auxiliary proteins and single-strand binding proteins, searches for and binds to the target homology domain. This results in the formation of a D-loop region at the homologous site, initiating strand exchange. Once recombinase dissociates, polymerase binds to the 3' end of the primer, initiating chain extension. The kit also utilizes exonuclease activity and specific molecular probes for real-time monitoring of the amplification process through fluorescence detection equipment.
High Sensitivity
Designed for precise RNA amplification, ensuring accurate detection.
Fast
The reaction completes in just 20 minutes, offering quick amplification.
Stable
The dry powder format simplifies handling and storage.
Wide Compatibility
Compatible with a range of fluorescence quantitative PCR instruments, constant temperature fluorescence amplifiers, and other fluorescence detection devices.
For optimal performance, primers should be 30–35 bp in length. Shorter primers can affect amplification speed and sensitivity. The primers are designed to avoid secondary structures that may hinder the amplification process. The recommended amplicon length is between 150–500 bp.
The probe sequence does not overlap with the specific primer recognition site, the length is 46-52 nt, and the sequence avoids palindromic sequence, internal secondary structure and continuous repeated bases.There are four modification sites in the probe:a dSpacer (tetrahydrofuran, THF)is marked in the middle of about 30-35 nt away from the 5' end as the recognition site for exonuclease; a fluorescent group is marked upstream of the THF site , a quenching group is labeled downstream, and the distance between the two groups is 2-4 nt; THF is about 15 nt away from the 3' end, and the 3' end is labeled with a blocking group, such as an amine group, a phosphate group, or a C3- Spacer.
|
Composition |
Content |
|
A buffer |
1.6 mL×1Tube |
|
B buffer |
150 μL×1Tube |
|
Reagent |
48T |
|
Guide Manual |
1Copy |
Note: Considering the problem of nucleic acid degradation, RNA series products do not provide positive control templates and primer probes.
- Specifications:48 T/pack
- Storage Conditions: Store at ≤-20ºC in a constant temperature environment. Keep away from light and avoid heavy pressure.
- Shelf Life: 14 months.
- Self-designed primers/probes must be added for MIRA project development.
- The kit is available in two types - DNA and RNA. The RNA type includes reverse transcriptase, allowing amplification with RNA, dsDNA, and ssDNA as templates. The DNA type can only amplify with dsDNA as the template.
- Available in three types - Basic, Fluorescent, and Test Strip. Choose the appropriate kit based on your needs and use the corresponding primers/probes.
http://www.amp-futurebio.com/